perinatologyРоссийский вестник перинатологии и педиатрииRossiyskiy Vestnik Perinatologii i Pediatrii (Russian Bulletin of Perinatology and Pediatrics)1027-40652500-2228Ltd. “The National Academy of Pediatric Science and Innovation”10.21508/1027-4065-2020-65-5-169-175perinatology-1254Research ArticleК XVII РОССИЙСКОМУ КОНГРЕССУ «ПЕДИАТРИЯ И ДЕТСКАЯ ХИРУРГИЯ В ПРИВОЛЖСКОМ ФЕДЕРАЛЬНОМ ОКРУГЕ». ОРИГИНАЛЬНЫЕ СТАТЬИTO THE XVI RUSSIAN CONGRESS «PEDIATRICS AND PEDIATRIC SURGERY IN THE PRIVOLZHSKY FEDERAL DISTRICT». ORIGINAL ARTICLESИсследование потокзависимой вазодилатации плечевой артерии и других маркеров функции эндотелия у детей с семейной гиперхолестеринемиейThe study of flow-mediated vasodilation of the brachial artery and other markers of endothelial function in children with familial hypercholesterolemiahttps://orcid.org/0000-0001-5576-5279ГалимоваЛ. Ф.GalimovaL. F.

Галимова Лилия Фаридовна — кандидат медицинских наук, врач Детской республиканской клинической больницы, ассистент кафедры госпитальной педиатрии Казанского ГМУ

Kazan

lilu1@inbox.ruhttps://orcid.org/0000-0002-6662-3548СадыковаД. И.SadykovaD. I.

Садыкова Динара Ильгизаровна — доктор медицинских наук, заведующий кафедрой госпитальной педиатрии

https://orcid.org/0000-0002-5273-6859ЛеонтьеваИ. В.LeontyevaI. V.

Леонтьева Ирина Викторовна — доктор медицинских наук, профессор, главный научный сотрудник отдела детской кардиологии и аритмологии НИКИПИ им. академика Ю.Е. Вельтищева.

125412, Москва, ул. Талдомская, д. 2

Moscow

https://orcid.org/0000-0002-1732-7443СластниковаЕ. С.SlastnikovaE. S.

Сластникова Евгения Сергеевна — врач Детской республиканской клинической больницы, аспирант кафедры госпитальной педиатрии Казанского ГМУ.

420138 Казань, ул. Оренбургский тракт, д. 140

Kazan

https://orcid.org/0000-0002-5505-4984КурмаеваН. Ш.KurmaevaN. Sh.

Курмаева Найра Шафкатовна — ассистент кафедры клинической иммунологии с аллергологией.

420012 Казань, ул. Бутлерова, д. 49

ГАУЗ Детская республиканская клиническая больница Минздрава республики Татарстан; ФГБОУ ВО Казанский государственный медицинский университет Минздрава РоссииРоссияRepublican Children’s Hospital; Kazan State Medical UniversityRussian FederationФГБОУ ВО Казанский государственный медицинский университет Минздрава РоссииРоссияKazan State Medical UniversityRussian FederationОСП Научно-исследовательский клинический институт педиатрии им. академика Ю.Е. Вельтищева, ФГАОУ ВО РНИМУ им. Н.И. Пирогова Минздрава РоссииРоссияVeltischev Research and Clinical Institute for Pediatrics of the Pirogov Russian National Research Medical UniversityRussian Federation202021112020655169175Copyright © Ltd. “The National Academy of Pediatric Science and Innovation”, 20202020Ltd. “The National Academy of Pediatric Science and Innovation”Ltd. “The National Academy of Pediatric Science and Innovation”https://www.ped-perinatology.ru/jour/about/submissions#copyrightNoticehttps://www.ped-perinatology.ru/jour/article/view/1254Цель исследования

Цель исследования. Изучить состояние функции эндотелия у детей с семейной гиперхолестеринемией путем оценки потокзависимой вазодилатации плечевой артерии и уровня оксида азота и эндотелина-1 в сыворотке крови.

Характеристика детей и методы исследования. В исследовании участвовали 62 ребенка, из них 30 детей (средний возраст 10,3±2,8 года) с диагнозом «семейная гиперхолестеринемия, гетерозиготная форма», не принимавшие липидснижающую терапию (основная группа); 32 здоровых ребенка (средний возраст 10,3±2,8 года) составили контрольную группу. Эндо-телин-1 и оксид азота в сыворотке крови определяли методом иммуноферментного анализа, потокзависимую вазодилатацию плечевой артерии — на аппарате Philips Affinity 70 (Release 3,0.3, Philips Healthcare, США).

Результаты

Результаты. Анализ уровня эндотелина-1 не выявил статистически значимых различий по этому показателю между здоровыми детьми и пациентами с семейной гиперхолестеринемией (0,213 [0,012; 0,368] и 0,227 [0,15; 0,315] пмоль/л соответственно; p=0,062). Уровень оксида азота у здоровых детей составил 33,6 [20—51] мкмоль/л, в то время как при семейной гиперхолестеринемии он был статистически значимо выше — 103,7 [48—152] мкмоль/л (р=0,001). Потокзависимая вазодилатация плечевой артерии у здоровых детей составила 9,82 [7,8; 11,2] %, при семейной гиперхолестеринемии этот показатель имел тенденцию к снижению — 9,46 [6,5; 10,8] % (р=0,742).

Заключение

Заключение. Исследование функции эндотелия у детей с семейной гиперхолестеринемией в возрасте 7—11 лет выявило увеличение концентрации оксида азота по сравнению с таковым у здоровых сверстников, вероятно, компенсаторного характера. Потокзависимая вазодилатация плечевой артерии и уровень эндотелина-1 достоверно не отличались от аналогичных показателей контрольной группы. Ввиду возможного кумулятивного действия липопротеинов низкой плотности необходимо мониторирование функции эндотелия у детей с семейной гиперхолестеринемией в динамике.

Objective

Objective. To study the state of endothelial function in children with familial hypercholesterolemia by evaluating the flow-dependent vasodilation of the brachial artery and serum nitric oxide and endothelin-1 levels.

Children characteristics and research methods. The study included total 62 children: 30 children (mean age 10,3 years ± 2,8) with a heterozygous form of familial hypercholesterolemia, not taking lipid-lowering therapy (main group), and 32 healthy children (mean age 10,3 years ± 2,8) in the control group. The content of endothelin-1 and nitric oxide in blood serum were determined by enzyme immunoassay. Flow-dependent vasodilation of brachial artery was measured using Philips Affinity 70 (Release 3,0.3, Philips Healthcare, USA).

Results

Results. The analysis of endothelin-1 level did not reveal statistically significant differences between healthy children and patients with familial hypercholesterolemia (0,213 [0,012; 0,368] and 0,227 [0,15; 0,315] pmol/l accordingly; p=0,062). The level of nitrogen oxide in healthy children was 33,6 [20—51] pmol/l, while patients with familial hypercholesterolemia had statistically significantly higher level of nitrogen oxide — 103,7 [48—152] pmol/l, p=0,001). Flow-dependent brachial artery vasodilation in healthy children was 9,82 [7,8; 11,2]% and in patients with familial hypercholesterolemia this indicator was decreased — 9,46 [6,5; 10,8]% (p=0,742).

Conclusion

Conclusion. A study of endothelial function in children with familial hypercholesterolemia aged 7—11 years revealed an increase in the concentration of nitrogen oxide relative to healthy patients, probably of a compensatory nature. The flow-dependent vasodilation of the brachial artery and the level of endothelin-1 did not significantly differ from the control group. Due to possible cumulative effect of low-density proteins, it is necessary to monitor endothelial function in children with familial hypercholesterolemia in dynamics.

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The authors declare that there are no conflicts of interest present.