perinatologyРоссийский вестник перинатологии и педиатрииRossiyskiy Vestnik Perinatologii i Pediatrii (Russian Bulletin of Perinatology and Pediatrics)1027-40652500-2228Ltd. “The National Academy of Pediatric Science and Innovation”perinatology-141Research ArticleАЛЛЕРГОЛОГИЯALLERGOLOGYСодержание антимикробных пептидов в сыворотке у детей с атопическим дерматитомSerum levels of antimicrobial peptides in children with atopic dermatitisЕрмолаеваЕ. И.ErmolaevaE. I.

асп. отделения иммунологии и клинической иммунологии НИКИ педиатрии РНИМУ им. Н.И. Пирогова

ВиноградоваТ. В.VinogradovaT. V.

к.м.н., в.н.с. лаборатории общей патологии того же учреждения 125412 Москва, ул.Талдомская, д. 2

ПампураА. Н.PampuraA. N.

д.м.н., рук. указанного отделения

ОСП «Научно-исследовательский клинический институт педиатрии» ГБОУ ВПО РНИМУ им. Н.И. Пирогова, МоскваРоссияResearch Clinical Institute of Pediatrics, N.I. Pirogov Russian National Research Medical University, MoscowRussian Federation2015130320166048992Copyright © Ltd. “The National Academy of Pediatric Science and Innovation”, 20162016Ltd. “The National Academy of Pediatric Science and Innovation”Ltd. “The National Academy of Pediatric Science and Innovation”https://www.ped-perinatology.ru/jour/about/submissions#copyrightNoticehttps://www.ped-perinatology.ru/jour/article/view/141

Цель работы: установить взаимосвязь уровня циркулирующих антимикробных пептидов — кателицидина (LL-37), альфа-дефензинов (HNP 1-3) в сыворотке крови с клиническими проявлениями атопического дерматита у детей. В исследование были включено 70 пациентов в возрасте от 5 мес до 17 лет с атопическим дерматитом. Уровень LL-37 и HNP1-3 определяли с помощью иммуноферментного анализа. По результатам исследования уровень HNP1-3 был выше у детей со средней тяжестью заболевания в сравнении с детьми с легкой степенью атопического дерматита. Концентрация LL-37 в сыворотке крови не зависела от индекса тяжести дерматита SCORAD. Однако уровень этого пептида был связан с выраженностью отдельных клинических проявлений заболевания. Концентрация LL-37 была повышена при площади поражения более 50% поверхности тела; уровень LL-37 возрастал с увеличением интенсивности экскориаций, лихенификации и зуда. В то же время по мере нарастания интенсивности корок/мокнутия от легкой до умеренной степени уровень LL-37 увеличивался и резко снижался при максимальной ее выраженности. Установленная взаимосвязь между концентрацией антимикробных пептидов и клиническими проявлениями атопического дерматита свидетельствует о патогенетической роли LL-37 и HNP1—3 в развитии данного заболевания.

Objective: to establish a relationship of the serum levels of the antimicrobial peptides (AMP) cathelicidin (LL-37) and a-defensins (HNP 1-3) to the clinical manifestations of atopic dermatitis (AD) in children. Subjects and methods. The investigation enrolled 70 patients aged 5 months to 17 years with AD. LL-37 and HNP1-3 levels were determined by enzyme immunoassay. Results. The level of HNP 1-3 was found to be higher in children with moderate AD than in those with its mild form. The serum concentration of LL-37 was independent of the severity scoring of atopic (SCORAD) index. However, the level of this peptide was associated with the magnitude of clinical manifestations of the disease. The concentration of LL-37 was increased with an injury over 50% of the body surface; its level rose with the higher intensity of excoriations, lichenification, and pruritus. At the same tone, as the intensity of crusts/oozing lesion increased from mild to moderate, LL-37 rose and drastically decreased at their highest intensity levels. Conclusion. The established correlation between AMP concentration and clinical manifestations of AD suggests that LL-37 and HNP1-3 play a role in the pathogenesis of this disease.

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The authors declare that there are no conflicts of interest present.