perinatologyРоссийский вестник перинатологии и педиатрииRossiyskiy Vestnik Perinatologii i Pediatrii (Russian Bulletin of Perinatology and Pediatrics)1027-40652500-2228Ltd. “The National Academy of Pediatric Science and Innovation”10.21508/1027-4065-2023-68-3-41-45perinatology-1824Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESДиагностическое значение структур межклеточного матрикса при бронхиальной астме у детейDiagnostic significance of the extracellular matrix structures in bronchial asthma in childrenhttps://orcid.org/0000-0002-9231-4407СтройковаТ. Р.StroykovaT. R.

Стройкова Татьяна Равильевна - к.м.н., доц. кафедры факультетской педиатрии

414000 Астрахань, ул. Бакинская, д. 121

Astrakhan

mega.astor@mail.ruhttps://orcid.org/0000-0002-1672-675XБашкинаО. А.BashkinaO. A.

Башкина Ольга Александровна - д.м.н., проф., зав. кафедрой факультетской педиатрии

414000 Астрахань, ул. Бакинская, д. 121

Astrakhan

https://orcid.org/0000-0003-0547-3686ГеппеН. А.GeppeN. A.

Геппе Наталья Анатольевна - д.м.н., проф., зав. кафедрой детских болезней Клинического института детского здоровья им. Н.Ф. Филатова

119435 Москва, ул. Большая Пироговская, д. 19, стр. 1

Moscow

ФГАОУ ВО «Астраханский государственный медицинский университет» Минздрава РоссииРоссияAstrakhan State Medical UniversityRussian FederationФГАОУ ВО «Первый Московский государственный медицинский университет им. И.М. Сеченова» Минздрава России (Сеченовский университет)РоссияSechenov First Moscow State Medical University (Sechenov University)Russian Federation2023140720236834145Copyright © Ltd. “The National Academy of Pediatric Science and Innovation”, 20232023Ltd. “The National Academy of Pediatric Science and Innovation”Ltd. “The National Academy of Pediatric Science and Innovation”https://www.ped-perinatology.ru/jour/about/submissions#copyrightNoticehttps://www.ped-perinatology.ru/jour/article/view/1824

Межклеточный матрикс при бронхиальной астме задействован в процессах структурного изменения, которые имеют как обратимый, так и необратимый характер. Диагностическую значимость могут иметь маркеры дисфункции эндотелия, показатели структур внеклеточного матрикса.Цель исследования. Изучение клинического значения маркеров коллагеногенеза и плазменного эндотелина-1 при бронхиальной астме у детей в зависимости от тяжести и длительности заболевания.Материалы и методы. Обследованы 268 пациентов в возрасте от 2 лет 11 мес до 17 лет 11 мес с установленным диагнозом бронхиальная астма различной степени тяжести. Методом иммуноферментного анализа (ИФА) определены уровни в сыворотке крови следующих показателей: антител к коллагенам I и III типов, плазменного эндотелина.Результаты. Определено статистически значимое повышение содержания фактора роста фибробластов, антител к коллагену I типа у детей с бронхиальной астмой тяжелой степени.Заключение. Данные показатели служат диагностическим маркером бронхиальной астмой тяжелой степени.

The intercellular matrix in bronchial asthma is involved in both reversible and irreversible processes of structural change. Markers of endothelial disorders, indicators of extracellular matrix structures may have diagnostic significance.Purpose. To study the clinical significance of markers of collagen genesis and plasma endothelin-1 in bronchial asthma in children, depending on the severity and duration of the disease.Material and methods. 268 patients aged from 2 years 11 months to 17 years 11 months with an established diagnosis of bronchial asthma with varying degrees of severity were examined. The ELISA method determined the serum levels of the following indicators: antibodies to type I and type III collagen, plasma endothelin.Results. There was a significantly significant increase in fibroblast growth factor, type I collagen antibodies in children with severe bronchial asthma.Conclusion. These indicators are a diagnostic marker for a severe bronchial asthma.

детибронхиальная астмавоспалениемежклеточный матрикссhildrenbronchial asthmainflammationintercellular matrixReferencesБэй-Дженсен А., И Дж. М.Б. Дженовезе Ф., Зибур А.С., Нильсен М., Лиминг Д.Дж. Биохимия коллагенов, ламининов и эластина. Aкадемик Пресс, 2016; 203–233.Be'j-Dzhensen A., I Dzh. M.B., Dzhenoveze F., Zibur A.S., Nil'sen M., Liming D.Dzh. Biochemistry of collagens, laminins and elastin, Academic Press, 2016; 203–233. (in Russ.)Robert J., Dhiren F., Patel F. Zooming into the Matrix: Using Nonlinear Optical Microscopy to Visualize Collagen Remodeling in Asthmatic Airways Am J Respir Crit Care Med 2019; 200(4): 403–405. DOI: 10.1164/rccm.201904–0722EDRobert J., Dhiren F., Patel F. Zooming into the Matrix: Using Nonlinear Optical Microscopy to Visualize Collagen Remodeling in Asthmatic Airways Am J Respir Crit Care Med 2019; 200(4): 403–405. DOI: 10.1164/rccm.201904–0722EDBurgess J., Mauad T., Tjin G., Karlsson J., Westergren-Thorsson G. The extracellular matrix–The under-recognized element in lung disease? J Pathol 2016; 240: 397–409. DOI: 10.1002/path.4808Burgess J., Mauad T., Tjin G., Karlsson J., Westergren-Thorsson G. The extracellular matrix–The under-recognized element in lung disease? J Pathol 2016; 240: 397–409. DOI: 10.1002/path.4808Halfter W., Oertle P., Monnier C., Camenzind L., Reyes-Lua M., Hu H. et al. New concepts in basement membrane biology. FEBS 2015; 282: 4466–4479. DOI: 10.1111/febs.13495Halfter W., Oertle P., Monnier C., Camenzind L., Reyes-Lua M., Hu H. et al. New concepts in basement membrane biology. FEBS 2015; 282: 4466–4479. DOI: 10.1111/febs.13495Le B., Macdonald B., Kalluri R. Structure and Function of Basement Membranes. Exp Biol Med 2007; 232: 1121–1129. DOI: 10.3181/0703-MR-72Le B., Macdonald B., Kalluri R. Structure and Function of Basement Membranes. Exp Biol Med 2007; 232: 1121–1129. DOI: 10.3181/0703-MR-72Faffe D., Zin W. Lung Parenchymal Mechanics in Health and Disease. Physiol Rev 2009; 89: 759–775. DOI: 10.1152/physrev.00019.2007Faffe D., Zin W. Lung Parenchymal Mechanics in Health and Disease. Physiol Rev 2009; 89: 759–775. DOI: 10.1152/physrev.00019.2007Booth A., Hadley R., Cornett, A., Dreffs A., Matthes S., Tsui J. et al. Acellular Normal and Fibrotic Human Lung Matrices as a Culture System for In Vitro Investigation Am J Respir Crit Care Med 2012; 186: 866–876. DOI: 10.1164/rccm.201204–0754OCBooth A., Hadley R., Cornett, A., Dreffs A., Matthes S., Tsui J. et al. Acellular Normal and Fibrotic Human Lung Matrices as a Culture System for In Vitro Investigation Am J Respir Crit Care Med 2012; 186: 866–876. DOI: 10.1164/rccm.201204–0754OCBurgstaller G., Oehrle B., Gerckens M., White E.S., Schiller H.B., Eickelberg O. The instructive extracellular matrix of the lung: Basic composition and alterations in chronic lung disease Eur Respir J 2017; 50: 160–180. DOI: 10.1183/13993003.01805–2016Burgstaller G., Oehrle B., Gerckens M., White E.S., Schiller H.B., Eickelberg O. The instructive extracellular matrix of the lung: Basic composition and alterations in chronic lung disease Eur Respir J 2017; 50: 160–180. DOI: 10.1183/13993003.01805–2016Gu B., Madison M., Corry D., Kheradmand F. Matrix remodeling in chronic lung diseases. Matrix Biol 2018; 73: 52–63. DOI: 10.1016/j.matbio.2018.03.012Gu B., Madison M., Corry D., Kheradmand F. Matrix remodeling in chronic lung diseases. Matrix Biol 2018; 73: 52–63. DOI: 10.1016/j.matbio.2018.03.012Emmanuel T. Special Issues Modeling Extracellular Matrix-Cell Interactions in Lung Repair and Chronic Disease. J Cells 2021; 8: 21–45. DOI: 10.3390/10082145Emmanuel T. Special Issues Modeling Extracellular Matrix-Cell Interactions in Lung Repair and Chronic Disease. J Cells 2021; 8: 21–45. DOI: 10.3390/10082145Hackett T., Vriesde N., Al-Fouadi M., Mostaco-Guidolin L., Maftoun D., Hsieh A. et al. Cells 2022; 11(2): 185. DOI: 10.3390Hackett T., Vriesde N., Al-Fouadi M., Mostaco-Guidolin L., Maftoun D., Hsieh A. et al. Cells 2022; 11(2): 185. DOI: 10.3390Yick C., Ferreira D., Annoni R., von der Thusen J., Kunst P., Bel E. et al. Extracellular matrix in airway smooth muscle is associated with dynamics of airway function in asthma. Allergy 2012; 67: 552–559. DOI: 10.1111/j.1398–9995.2011.02773Yick C., Ferreira D., Annoni R., von der Thusen J., Kunst P., Bel E. et al. Extracellular matrix in airway smooth muscle is associated with dynamics of airway function in asthma. Allergy 2012; 67: 552–559. DOI: 10.1111/j.1398–9995.2011.02773James A., Maxwell P., Pearce-Pinto G., Elliot J., Carroll N. The Relationship of Reticular Basement Membrane Thickness to Airway Wall Remodeling in Asthma. Am J Respir Crit Care Med 2002; 166: 1590–1595. DOI: 10.1164/rccm.2108069James A., Maxwell P., Pearce-Pinto G., Elliot J., Carroll N. The Relationship of Reticular Basement Membrane Thickness to Airway Wall Remodeling in Asthma. Am J Respir Crit Care Med 2002; 166: 1590–1595. DOI: 10.1164/rccm.2108069Sagliani S., Molyneux C., Gong H., Rogers A., Malmstrom A., Pelkonen A. et al. Ultrastructure of the reticular basement membrane in adults, children and infants with asthma Eur Respir J 2006; 28(3): 505–512. DOI: 10.1183/09031936.06.00056405Sagliani S., Molyneux C., Gong H., Rogers A., Malmstrom A., Pelkonen A. et al. Ultrastructure of the reticular basement membrane in adults, children and infants with asthma Eur Respir J 2006; 28(3): 505–512. DOI: 10.1183/09031936.06.00056405Leila B., Mostaço-Guidolin L., Emmanuel T., Jari U., Hajimohammadi S., Fouadi M., Xian L. Defective Fibrillar Collagen Organization by Fibroblasts Contributes to Airway Remodeling in Asthma Am J Respir Crit Care Med 2019; 200(4): 431–443. DOI: 10.1164/rccm.201810–1855OCLeila B., Mostaço-Guidolin L., Emmanuel T., Jari U., Hajimohammadi S., Fouadi M., Xian L. Defective Fibrillar Collagen Organization by Fibroblasts Contributes to Airway Remodeling in Asthma Am J Respir Crit Care Med 2019; 200(4): 431–443. DOI: 10.1164/rccm.201810–1855OCElias J.A., Zhu Z., Chupp G., Homer R. Airway remodeling in asthma. J. Clin. Investig 1999; 104: 1001–1006. DOI: 10.1172/JCI8124Elias J.A., Zhu Z., Chupp G., Homer R. Airway remodeling in asthma. J. Clin. Investig 1999; 104: 1001–1006. DOI: 10.1172/JCI8124Zeiger R.S., Dawsonb C., Weiss S. Relationships between duration of asthma and asthma severity among children in the Childhood Asthma Management Program (CAMP). J Allergy Clin Immunol 1999; 103: 376–386. DOI: 10.1016/s0091–6749(99)70460–4Zeiger R.S., Dawsonb C., Weiss S. Relationships between duration of asthma and asthma severity among children in the Childhood Asthma Management Program (CAMP). J Allergy Clin Immunol 1999; 103: 376–386. DOI: 10.1016/s0091–6749(99)70460–4Mckay O., Hogg J. The contribution of airway structure to early childhood asthma. Med J Aust 2002; 177: S45–S47. DOI: 10.5694/j.1326–5377.2002.tb04852.xMckay O., Hogg J. The contribution of airway structure to early childhood asthma. Med J Aust 2002; 177: S45–S47. DOI: 10.5694/j.1326–5377.2002.tb04852.xBoulet L., La Violette M., Turcotte H., Cartier A., Dugas M., Malo J.-L., Boutet M. Bronchial subepithelial fibrosis correlates with airway responsiveness to methacholine. Chest 1997; 112: 45–52. DOI: 10.1378/112.1.45Boulet L., La Violette M., Turcotte H., Cartier A., Dugas M., Malo J.-L., Boutet M. Bronchial subepithelial fibrosis correlates with airway responsiveness to methacholine. Chest 1997; 112: 45–52. DOI: 10.1378/112.1.45Araujo B.B., Dolhnikoff M., da Silva L.F.F., Elliot J., Lindeman J.H.N., Ferreira D. et al. Extracellular matrix components and regulators in the airway smooth muscle in asthma. Eur Respir J 2008; 32: 61–69. DOI: 10.1183/09031936.00147807Araujo B.B., Dolhnikoff M., da Silva L.F.F., Elliot J., Lindeman J.H.N., Ferreira D. et al. Extracellular matrix components and regulators in the airway smooth muscle in asthma. Eur Respir J 2008; 32: 61–69. DOI: 10.1183/09031936.00147807Mostaço-Guidolin L.B., Yang C.X., Hackett T.L. Pulmonary vascular remodeling is an early feature of fatal and non-fatal asthma. Am. J. Respir. Cell Mol Biol 2021; 65: 114–118. DOI: 10.1165/rcmb.2020–0339LEMostaço-Guidolin L.B., Yang C.X., Hackett T.L. Pulmonary vascular remodeling is an early feature of fatal and non-fatal asthma. Am. J. Respir. Cell Mol Biol 2021; 65: 114–118. DOI: 10.1165/rcmb.2020–0339LEPayne D., Rogers A., Adelroth E., Bandi V., Guntupalli K.K., Bush A., Jeffery P.K. Early thickening of the reticular basement membrane in children with difficult asthma. Am J Respir Crit Care Med 2003; 167: 78–82. DOI: 10.1164/rccm.200205–414OCPayne D., Rogers A., Adelroth E., Bandi V., Guntupalli K.K., Bush A., Jeffery P.K. Early thickening of the reticular basement membrane in children with difficult asthma. Am J Respir Crit Care Med 2003; 167: 78–82. DOI: 10.1164/rccm.200205–414OCMostaço-Guidolin L., Osei E., Ullah J., Hajimohammadi S., Fouadi M., Li X. et al. Defective Fibrillar Collagen Organization by Fibroblasts Contributes to Airway Remodeling in Asthma. Am J Respir Crit Care Med 2019; 200: 431–443. DOI: 10.1164/rccm.201810–1855OCMostaço-Guidolin L., Osei E., Ullah J., Hajimohammadi S., Fouadi M., Li X. et al. Defective Fibrillar Collagen Organization by Fibroblasts Contributes to Airway Remodeling in Asthma. Am J Respir Crit Care Med 2019; 200: 431–443. DOI: 10.1164/rccm.201810–1855OCСтройкова Т.Р., Григанов В.И. Проблема хронического воспаления при бронхиальной астме у детей. Астраханский медицинский журнал 2011; 3: 52–55.Stroikova T.R., Griganov V.I. The problem of chronic inflammation in bronchial asthma in children. Astrakhanskii meditsinskii zhurnal 2011; 3: 52–55. (in Russ.)Global Initiative for Asthma. Global strategy for asthma management and prevention vention. Updated 2021. Available from: https://ginasthma.org/ginareports / Ссылка активна на 02.05.2023.Global Initiative for Asthma. Global strategy for asthma management and prevention vention. Updated 2021. Available from: https://ginasthma.org/ginareports / Ссылка активна на 02.05.2023.Pałgan K., Bartuzi Z. Int J Immunopathol Pharmacol 2015; 28(3): 415–420. DOI: 10.1177/0394632015580907Pałgan K., Bartuzi Z. Int J Immunopathol Pharmacol 2015; 28(3): 415–420. DOI: 10.1177/0394632015580907Hoshino M., Nakamura Y., Sim J. Expression of growth factors and remodeling of the airway wall in bronchial asthma. Thorax 1998; 53(1): 21–27. DOI: 10.1136/thx.53.1.21Hoshino M., Nakamura Y., Sim J. Expression of growth factors and remodeling of the airway wall in bronchial asthma. Thorax 1998; 53(1): 21–27. DOI: 10.1136/thx.53.1.21Целуйко С.С., Красавина Н.П. Иммуноцитохимическая характеристика типов коллагена соединительной ткани биоптатов бронхов больных с дисплазией органов дыхания. Бюллетень физиологии и патологии дыхания 2010; 38: 36–42.Tseluiko S.S., Krasavina N.P. Immunocytochemical characterization of connective tissue collagen types in bronchial biopsy specimens from patients with respiratory dysplasia. Byulleten’ fiziologii i patologii dykhaniya 2010; 38: 36–42. (in Russ.)

The authors declare that there are no conflicts of interest present.