perinatologyРоссийский вестник перинатологии и педиатрииRossiyskiy Vestnik Perinatologii i Pediatrii (Russian Bulletin of Perinatology and Pediatrics)1027-40652500-2228Ltd. “The National Academy of Pediatric Science and Innovation”10.21508/1027-4065-2016-61-4-104-107perinatology-361Research ArticleАЛЛЕРГОЛОГИЯALLERGOLOGYОсобенности сенсибилизации к рекомбинантным аллергенам арахиса у детей с анафилаксиейSpecific features of sensitization to recombinant peanut allergens in children with anaphylaxisШуляеваА. М.ShulyaevaA. M.

аспирантка отдела аллергологии и иммунологии НИКИ педиатрии им. академика Ю. Е. Вельтищева

ПампураА. Н.PampuraA. N.

д.м.н., рук. того же отдела

ОкуневаТ. С.OkunevaT. S.

ст. научн. сотр. научно-исследовательской лаборатории общей патологии 125412 Москва, ул. Талдомская, д. 2

ОСП «Научно-исследовательский клинический институт педиатрии им. академика Ю. Е. Вельтищева» ГБОУ ВПО РНИМУ им. Н. И. Пирогова Минздрава РФ, МоскваРоссияAcademician Yu. E. Veltishchev Research Clinical Institute of Pediatrics N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, MoscowRussian Federation201606092016614104107Copyright © Ltd. “The National Academy of Pediatric Science and Innovation”, 20162016Ltd. “The National Academy of Pediatric Science and Innovation”Ltd. “The National Academy of Pediatric Science and Innovation”https://www.ped-perinatology.ru/jour/about/submissions#copyrightNoticehttps://www.ped-perinatology.ru/jour/article/view/361

Использование молекулярной аллергодиагностики позволяет не просто выявить сенсибилизацию к аллергену арахиса в целом, но и установить, к какому конкретному аллергену синтезированы специфические IgE. Целью настоящего исследования явилось выяснение связи сенсибилизации к определенным молекулам арахиса и аллергической реакции в виде анафилаксии при употреблении продукта. Иммунофлюоресцентным методом (Immuno CAP 100 Phadia AB, Швейцария) у пациентов было проведено определение специфических IgE к цельному аллергену арахиса, а также его компонентам (Ara h1. Ara h2, Ara h3, Ara h8, Ara h9). У детей с выявленной анафилаксией к аллергену арахиса достоверно чаще выявлялась одновременно сенсибилизация к рекомбинантным аллергенам Ara h 8 и Ara h9 (р=0,019). Выявленное сочетание сенсибилизации к рекомбинантным аллергенам арахиса Ara h8 и Ara h9 указывает на высокую вероятность манифестации аллергической реакции в виде анафилаксии. Определение специфических IgE к белкам арахиса позволит оптимизировать тактику ведения пациентов.

Molecular allergy diagnosis makes it possible not only to identify allergic sensitization to peanut as a whole, but also to establish the specific allergen the specific IgE is synthesized to. The purpose of this study was to establish a relationship between sensitization to certain molecules of peanut and allergy as anaphylaxis after eating the product. An immunofluorescence assay (Immuno CAP 100 Phadia AB, Switzerland) was used to determine specific IgE to whole allergen in the peanut and its components (Ara h1, Ara h2, Ara h3, Ara h8, and h9 Ara). Children diagnosed with peanut-induced anaphylaxis were found to be significantly more sensitized to the recombinant allergens Ara h8 and Ara h9 (p=0.019). The detected combination of sensitization to the recombinant peanut allergen Ara h8 and Ara h9 indicates a high probability that an allergic reaction may manifest as anaphylaxis. Determination of specific IgE against peanut proteins will optimize patient management tactics.

детипищевая аллергиясенсибилизациярекомбинантные аллергеныарахискомпонентная диагностикаchildrenfood allergysensitizationrecombinant allergenspeanutcomponent-resolved diagnosisReferencesGrundy J., Matthews S., Bateman B. et al. Rising prevalence of allergy to peanut in children. Journal Allergy Clinical Immunology 2002; 110: 5: 784–789.Grundy J., Matthews S., Bateman B. et al. Rising prevalence of allergy to peanut in children. Journal Allergy Clinical Immunology 2002; 110: 5: 784–789.Colver A. Are the dangers of childhood food allergy exaggerated? Biochem Med J 2006; 333: 7566: 494–496.Colver A. Are the dangers of childhood food allergy exaggerated? Biochem Med J 2006; 333: 7566: 494–496.http://www.aafa.org/http://www.aafa.org/http://www.cdc.gov/http://www.cdc.gov/De Long J. H., Simpson K. H., Wambre E. et al. Ara h 1‑reactive T cells in individuals with peanut allergy. J Allergy Clin Immunol 2011; 127: 5: 1211–1218.De Long J. H., Simpson K. H., Wambre E. et al. Ara h 1‑reactive T cells in individuals with peanut allergy. J Allergy Clin Immunol 2011; 127: 5: 1211–1218.Mueller G. A., Gosavi R. A., PomesA. et al. Ara h 2: crystal structure and IgE binding distinguish two subpopulations of peanut allergic patients by epitope diversity. Allergy J 2011; 66: 7: 878–885.Mueller G. A., Gosavi R. A., PomesA. et al. Ara h 2: crystal structure and IgE binding distinguish two subpopulations of peanut allergic patients by epitope diversity. Allergy J 2011; 66: 7: 878–885.Dean T. P. Immunological responses in peanut allergy. Clinical Expert Allergy 1998; 28: 7–9.Dean T. P. Immunological responses in peanut allergy. Clinical Expert Allergy 1998; 28: 7–9.Scurlock A. M., Burks A. W. Peanut allergenicity. Allergy Asthma Immunol 2004; 93: 5: 8–12.Scurlock A. M., Burks A. W. Peanut allergenicity. Allergy Asthma Immunol 2004; 93: 5: 8–12.Koppelman S. J., Bruijnzeel-Koomen C. A., Hessing M. et al. Heat-induced conformational changes of Ara h 1, a major peanut allergen, do not affect its allergenic properties. J Biol Chem 1999; 274: 8: 4770–4777.Koppelman S. J., Bruijnzeel-Koomen C. A., Hessing M. et al. Heat-induced conformational changes of Ara h 1, a major peanut allergen, do not affect its allergenic properties. J Biol Chem 1999; 274: 8: 4770–4777.Klemans R. J., Otte D., Knol M. et al. The diagnostic value of specific IgE to Ara h2 to predict peanut allergy in children is comparable to a validated and updated diagnostic pradiction model. J Allergy Clin Immunol 2013; 131: 157–163.Klemans R. J., Otte D., Knol M. et al. The diagnostic value of specific IgE to Ara h2 to predict peanut allergy in children is comparable to a validated and updated diagnostic pradiction model. J Allergy Clin Immunol 2013; 131: 157–163.Ciprandi G., Pistorio A., Silvestri A. et al. Peanut anaphylaxis: the usefulness of molecular — based allergy diagnostics. J Allergy Clin Immunol 2015; 70: 129–130.Ciprandi G., Pistorio A., Silvestri A. et al. Peanut anaphylaxis: the usefulness of molecular — based allergy diagnostics. J Allergy Clin Immunol 2015; 70: 129–130.Asarnoy A., Nilson C., Lidholm et al. Peanut component Ara h8 sensitisation and toleranse to peanut. J Allergy Clin Immunol 2012; 130: 2: 468–472.Asarnoy A., Nilson C., Lidholm et al. Peanut component Ara h8 sensitisation and toleranse to peanut. J Allergy Clin Immunol 2012; 130: 2: 468–472.Шуляева А. М., Пампура А. Н., Окунева Т. С. и соавт. Клиническое значение компонентной диагностики к аллергенам арахиса у детей с аллергическими заболеваниями. Рос аллергол журн 2014; 6: 41–46. (Shuljaeva A. M., Pampura A. N., Okuneva T. S. et al. Clinical role of component diagnostics of peanut allergy among children with allergic diseases. Ros Аllergol ZHurn 2014; 6: 41–46.)Шуляева А. М., Пампура А. Н., Окунева Т. С. и соавт. Клиническое значение компонентной диагностики к аллергенам арахиса у детей с аллергическими заболеваниями. Рос аллергол журн 2014; 6: 41–46. (Shuljaeva A. M., Pampura A. N., Okuneva T. S. et al. Clinical role of component diagnostics of peanut allergy among children with allergic diseases. Ros Аllergol ZHurn 2014; 6: 41–46.)Agabriel C., Ghazouani O., Birnbaum J. et al. Ara h 2 and Ara h 6 sensitisationpredict peanut allergy in Mediterranean pediatric patient. Pediatr Allergy Immunol 2014; 11: 7.Agabriel C., Ghazouani O., Birnbaum J. et al. Ara h 2 and Ara h 6 sensitisationpredict peanut allergy in Mediterranean pediatric patient. Pediatr Allergy Immunol 2014; 11: 7.Klemans R., Otte D., Knol M. et al. The diagnostic value of specific IgE to Ara h 2 to predict peanut allergy in children is comparable to a validated and updated diagnostic prediction model. J Allergy Clin Immunol 2013; 131: 1: 157–163.Klemans R., Otte D., Knol M. et al. The diagnostic value of specific IgE to Ara h 2 to predict peanut allergy in children is comparable to a validated and updated diagnostic prediction model. J Allergy Clin Immunol 2013; 131: 1: 157–163.Dang T., Tang M., Choo S. et al. Increasing the accuracy of peanut allergy diagnosis by using Ara h 2. J Allergy Clin Immunol 2012; 129: 4: 1056–1063Dang T., Tang M., Choo S. et al. Increasing the accuracy of peanut allergy diagnosis by using Ara h 2. J Allergy Clin Immunol 2012; 129: 4: 1056–1063

The authors declare that there are no conflicts of interest present.