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Intestinal microbiome and modern methods of its study in children
https://doi.org/10.21508/1027-4065-2022-67-4-5-13
Abstract
Исследования кишечного микробиома в настоящее время вызывают большой интерес у клиницистов. Это связано с тем, что результаты проведенных исследований показывают тесную взаимосвязь кишечного микробиома с развитием различных заболеваний. В статье представлены современные методы отбора проб для исследований, охарактеризованы мультиомические методы исследования (метагеномика, метапротеомика, метатранскриптомика, метаболомика) и возможности их применения в клинической практике с указанием их преимуществ и недостатков. Описаны наиболее распространенные методы исследования (при этом особое внимание уделено изучению кишечного микробиома), в которых можно получить оптимально объективные результаты и точность вычислительного анализа данных. Эти исследования будут способствовать развитию персонализированной медицины, которая будет применяться в самых различных областях — от точной идентификации патогенных штаммов для целенаправленного лечения до тщательного мониторинга дисбаланса микробных сообществ при заболеваниях и до персонализированного и рационального плана манипуляций с микробиомом.
Keywords
дети,
кишечная микробиота,
микробиом,
метагеномика,
метатранскриптомика,
матапротеомика,
метаболомика
Supporting agencies: The study was supported by the Biocodex Microbiota Foundation, National Grant 2021, Russia.
About the Authors
G. V. Volynets
Veltischev Research and Clinical Institute for Pediatrics at the Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
Moscow
A. V. Nikitin
Veltischev Research and Clinical Institute for Pediatrics at the Pirogov Russian National Research Medical University;
Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
Moscow
T. A. Skvortsova
Veltischev Research and Clinical Institute for Pediatrics at the Pirogov Russian National Research Medical University;
Pirogov Russian National Research Medical University;
Morozov Children’s City Clinical Hospital
Russian Federation
Russian Federation
Moscow
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Review
For citations:
Volynets G.V., Nikitin A.V., Skvortsova T.A. Intestinal microbiome and modern methods of its study in children. Rossiyskiy Vestnik Perinatologii i Pediatrii (Russian Bulletin of Perinatology and Pediatrics). 2022;67(4):5-13. (In Russ.) https://doi.org/10.21508/1027-4065-2022-67-4-5-13
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