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Achievements and future prospects of molecular allergy diagnostics in pediatrics
https://doi.org/10.21508/1027-4065-2025-70-1-5-10
Abstract
Improvements in clinical approaches and innovative technological solutions in the field of molecular allergy diagnostics have fundamentally changed the tactics and strategy for managing children with allergic diseases. In particular, molecular allergy diagnostics is used to make decisions in cases of suspected food allergy; to assess the risks of life-threatening reactions; to optimize the elimination diet; to identify cross-reactivity; to diagnose inhalant allergies and determine their clinical significance;to provide optimal recommendations for reducing the exposure to inhalation allergens; to rationally select pharmacotherapy and allergen-specific immunotherapy; to predict the course of allergic disease, including the development of the atopic march. In addition, molecular allergy diagnostics is the foundation for establishing a system for preventing allergic diseases. In this article, we have tried to outline the main achievements and prospects of molecular allergy diagnostics application in pediatric practice.
Supporting agencies: The research work and the publication of its results had been carried out with the financial support from the Moscow City Government Grant for the Implementation of a Scientific and Practical Project in Medicine No. 0408–1.
About the Authors
A. N. Pampura
Veltischev Research and Clinical Institute for pediatrics and pediatric surgery at the Pirogov Russian National Research Medical University; Morozov Children’s City Hospital
Russian Federation
Russian Federation
Moscow
E. F. Zhukalina
Veltischev Research and Clinical Institute for pediatrics and pediatric surgery at the Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
Moscow
M. A. Morenko
Morozov Children’s City Hospital
Kazakhstan
Kazakhstan
Moscow
O. P. Ussenova
Astana medical university
Kazakhstan
Kazakhstan
Astana
References
1. Valenta R., Karaulov A., Niederberger V., Gattinger P., van Hage M., Flicker S., et al. Molecular aspects of allergens and allergy. Adv Immunol 2018; 138: 195–256. DOI: 10.1016/bs.ai.2018.03.002
2. Valenta R., Lidholm J., Niederberger V., Hayek B., Kraft D., Grönlund H. The recombinant allergen-based concept of component-resolved diagnostics and immunotherapy (CRD and CRIT). Clin Exp Allergy 1999; 29(7): 896–904. DOI: 10.1046/j.1365–2222.1999.00653.x
3. Jakob T., Forstenlechner P., Matricardi P., Kleine-Tebbe J. Molecular allergy diagnostics using multiplex assays: methodological and practical considerations for use in research and clinical routine: Part 21 of the Series Molecular Allergology. Allergo J Int 2015; 24: 320–332. DOI: 10.1007/s40629–015–0087–8
4. Ansotegui I.J., Melioli G., Canonica G.W., Caraballo L., Villa E., Ebisawa M. IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paper. World Allergy Organ J 2020; 13(2): 100080. DOI: 10.1016/j.waojou.2019.100080
5. Garib V., Trifonova D., Freidl R., Linhart B., Schlederer T., Douladiris N. et al. Milk Allergen Micro-Array (MAMA) for Refined Detection of Cow’s-Milk-Specific IgE Sensitization. Nutrients 2023; 15(10): 2401. DOI: 10.3390/nu15102401
6. Dramburg S., Hilger C., Santos A.F., de Las Vecillas L., Aalberse R.C., Acevedo N., et al. EAACI Molecular Allergology User’s Guide 2.0. Pediatr Allergy Immunol 2023; 34 Suppl 28: e13854. DOI: 10.1111/pai.13854. PMID: 37186333
7. Riggioni C., Ricci C., Moya B., Wong D., van Goor E., Bartha I. et al. Systematic review and meta-analyses on the accuracy of diagnostic tests for IgE-mediated food allergy. Allergy 2024; 79(2): 324–352. DOI: 10.1111/all.15939
8. Пампура А.Н., Есакова Н.В. Анафилаксия у детей. М.: Медпрактика-М, 2020; 368. [Pampura A.N., Esakova N.V. Anaphylaxis in children. Moscow: Medpraktika, 2020; 368. (in Russ.)
9. Golden D.B.K., Wang J., Waserman S., Akin C., Campbell R.L., Ellis A.K. et al. Anaphylaxis: A 2023 practice parameter update. Ann Allergy Asthma Immunol 2024; 132(2): 124–176. DOI: 10.1016/j.anai.2023.09.015
10. Tosca M.A., Schiavetti I., Olcese R., Trincianti C., Ciprandi G. Molecular Allergy Diagnostics in Children with Cow’s Milk Allergy: Prediction of Oral Food Challenge Response in Clinical Practice. J Immunol Res 2023; 2023: 1129449. DOI: 10.1155/2023/1129449
11. Esakova N.V., Pampura A.N. Features of food anaphylaxis in children arising from alternative routes of allergen intake. Pediatrija. Zhurnal im. G.N. Speranskogo. 2021; 100(2): 57–63. (in Russ.) DOI: 10.24110/0031–403X-2021–100–2–57–63
12. Fiocchi A., Bognanni A., Brożek J., Ebisawa M., Schünemann H. WAO DRACMA guideline group. World Allergy Organization (WAO) Diagnosis and Rationale for Action against Cow’s Milk Allergy (DRACMA) Guidelines update — I — Plan and definitions. World Allergy Organ J 2022; 15(1): 100609. DOI: 10.1016/j.waojou.2021.100609
13. Pampura A.N., Zhukalina E.F., Morenko M.A., Ussenova O.P. Modern approaches to the diagnosis and management of children with allergy to cow’s milk proteins. Ros vestn perinatol i pediatr 2023; 68:(2): 39–46. (in Russ.). DOI: 10.21508/1027– 4065–2023–68–2–39–46
14. Gómez T.E., Gratacós G.A.R., González J.O.M., Joyanes R.J.B., Palacios C.A, García R.R. Profilins and Food-Dependent Exercise-Induced Anaphylaxis. J Investig Allergol Clin Immunol 2021; 31(4): 349–350. DOI: 10.18176/jiaci.0650
15. Santos A.F., Riggioni C., Agache I., Akdis C.A., Akdis M., Alvarez-Perea A. et al. EAACI guidelines on the diagnosis of IgE-mediated food allergy. Allergy 2023; 78(12): 3057–3076. DOI: 10.1111/all.15902
16. Tedner S.G., Asarnoj A., Thulin H., Westman M., Konradsen J.R., Nilsson C. Food allergy and hypersensitivity reactions in children and adults-A review. J Intern Med 2022; 291(3): 283–302. DOI: 10.1111/joim.13422
17. Skypala I.J., Bartra J., Ebo D.G., Antje Faber M., Fernandez-Rivas M., Gomez F., et al. The diagnosis and management of allergic reactions in patients sensitized to non-specific lipid transfer proteins. Allergy 2021; 76: 2433–2446. DOI: 10.1111/all.14797
18. Bublin M., Breiteneder H. Cross-reactivities of non-homologous allergens. Allergy 2020; 75: 1019–1022. DOI: 10.1111/all.14120
19. Candreva A.M., Smaldini P.L., Cauerhff A., Petruccelli S., Docena G.H. A novel approach to ameliorate experimental milk allergy based on the oral administration of a short soy cross-reactive peptide. Food Chem 2021; 346: 128926. DOI: 10.1016/j.foodchem.2020.128926
20. Warm K., Lindberg A., Lundbäck B., Rönmark E. Increase in sensitization to common airborne allergens among adults — two population-based studies 15 years apart. Allergy Asthma Clin Immunol 2013; 9(1): 20. DOI: 10.1186/1710–1492–9–20
21. Sarzsinszky E., Lupinek C., Vrtala S., Huang H.J., Hofer G., Keller W. et al. Expression in Escherichia coli and Purification of Folded rDer p 20, the Arginine Kinase From Dermatophagoides pteronyssinus: A Possible Biomarker for Allergic Asthma. Allergy Asthma Immunol Res 2021; 13(1): 154–163. DOI: 10.4168/aair.2021.13.1.154
22. Zhukalina E.F., Pampura A.N., Surovenko T.N., Medvedeva A.D. Profiles of molecular sensitization to fur animal allergens in patients of the Russian Federation. Pediatrija 2024; 103(2): 17–26. (in Russ.) DOI:10.24110/0031–403X-2024–103–2–17–26
23. Alvaro-Lozano M., Akdis C.A., Akdis M., Alviani C., Angier E., Arasi S. et al. EAACI Allergen Immunotherapy User’s Guide. Pediatr Allergy Immunol 2020; 31 25(25): 1–101. DOI: 10.1111/pai.13189
24. Wickman M., Lupinek C., Andersson N., Belgrave D., Asarnoj A., Benet M. et al. Detection of IgE Reactivity to a Handful of Allergen Molecules in Early Childhood Predicts Respiratory Allergy in Adolescence. EBioMedicine 2017; 26: 91–99. DOI: 10.1016/j.ebiom.2017.11.009
25. Grönlund H., Saarne T., Gafvelin G., van Hage M. The major cat allergen, Fel d 1, in diagnosis and therapy. Int Arch Allergy Immunol. 2010;151(4): 265–274. DOI: 10.1159/000250435
26. Bjerg A., Winberg A., Berthold M., Mattsson L., Borres M.P., Rönmark E. A population-based study of animal component sensitization, asthma, and rhinitis in schoolchildren. Pediatr Allergy Immunol 2015; 26(6): 557–563. DOI: 10.1111/pai.12422
27. Hatzler L., Panetta V., Lau S., Wagner P., Bergmann R.L., Illi S. et al. Molecular spreading and predictive value of preclinical IgE response to Phleum pratense in children with hay fever. J Allergy Clin Immunol 2012; 130(4): 894–901.e5. DOI: 10.1016/j.jaci.2012.05.053
28. Jensen-Jarolim E., Pacios L.F., Bianchini R., Hofstetter G., Roth-Walter F. Structural similarities of human and mammalian lipocalins, and their function in innate immunity and allergy. Allergy 2016; 71(3): 286–94. DOI: 10.1111/все.12797
29. Schoos A.M., Kattan J.D., Gimenez G., Sampson H.A. Sensitization phenotypes based on protein groups and associations to allergic diseases in children. J Allergy Clin Immunol 2016; 137(4): 1277–1280. DOI: 10.1016/j.jaci.2015.09.035
30. Steering Committee Authors; Review Panel Members. A WAO — ARIA — GA2LEN consensus document on molecular-based allergy diagnosis (PAMD@): Update 2020. World Allergy Organ J 2020; 13(2): 100091. DOI: 10.1016/j.waojou.2019.100091
Review
For citations:
Pampura A.N., Zhukalina E.F., Morenko M.A., Ussenova O.P. Achievements and future prospects of molecular allergy diagnostics in pediatrics. Rossiyskiy Vestnik Perinatologii i Pediatrii (Russian Bulletin of Perinatology and Pediatrics). 2025;70(1):5-10. (In Russ.) https://doi.org/10.21508/1027-4065-2025-70-1-5-10
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