Features of the immune system of children born to women with gestational diabetes mellitus
Abstract
Objective: to assess the state of the immune system in children born to women with gestational diabetes. Forty-two of umbilical cord blood samples from full-term infants, including 22 babies born to women with gestational diabetes mellitus (GDM) (a study group) and 20 born to those without this condition (a comparison group), were explored. Flow cytofluorometry was used to measure the counts of major T- and B- lymphocyte population, the level of expression of activation markers on monocytes (CD14+ HLA-DR+) and lymphocytes (CD25, CD69), as well as markers characterizing the functional maturity of cells (CD45R0, CD45RA) in both the total lymphocyte pool and T cell population. The serum levels of IL-1β, IL-4, IL-6, IL-8, IL-10, TNF-α, and IFN-γ were estimated by ELISA. The immune system of the infants born to women with GDM was established to be characterized by a decrease in the relative count of CD3+, CD4+ and CD3+CD45RA+ cells and in the expression of activation markers (CD25+, CD4+ CD25+ and CD14+ HLA-DR+) in conjunction with an increase in the absolute count of primed T lymphocytes and in the levels of natural killer cells and IL-8. This investigation provides a way of identifying a group at risk for postnatal infectious diseases. The decline in the indicators of innate and adaptive immunity in the umbilical cord blood may be a ground for ultrasonography in newborn babies born to women with GDM to detect thymic abnormalities.
About the Authors
G. N. Chistyakova
Ural Research Institute of Maternity and Infancy Care, Ministry of Health of the Russian Federation, Yekaterinburg
Russian Federation
Russian Federation
I. I. Remizova
Ural Research Institute of Maternity and Infancy Care, Ministry of Health of the Russian Federation, Yekaterinburg
Russian Federation
Russian Federation
I. A. Gazieva
Ural Research Institute of Maternity and Infancy Care, Ministry of Health of the Russian Federation, Yekaterinburg
Russian Federation
Russian Federation
V. A. Lyapunov
Ural Research Institute of Maternity and Infancy Care, Ministry of Health of the Russian Federation, Yekaterinburg
Russian Federation
Russian Federation
L. S. Ustyantseva
Ural Research Institute of Maternity and Infancy Care, Ministry of Health of the Russian Federation, Yekaterinburg
Russian Federation
Russian Federation
References
1. Килина А.В., Колесникова М.Б. Антенатальное развитие и течение адаптационного периода новорожденных, родившихся у матерей с гестационным сахарным диабетом. Вопр соврем педиат 2008; 7: 2: 111—113. (Kilina A.V., Kolesnikova M.B. Prenatal development and the adaptation period for infants born to mothers with gestational diabetes. Vopr sovrem pediat 2008; 7: 2: 111—113.)
2. Эсмурзиева З.И., Кузьменко Л.Г. Состояние здоровья детей от матерей, больных сахарным диабетом. The journal of scientific articles «Health & education millennium» 2014; 16: 1: 1—8. (Jesmurzieva Z.I., Kuz’menko L.G. The health status of children of mothers with diabetes. The journal of scientific articles «Health & education millennium» 2014; 16: 1: 1—8.)
3. Сиротина О.Б., Зубарева Е.А. Ультразвуковая оценка тимуса плодов и новорожденных от матерей с сахарным диабетом 1-го типа. Ультразвуковая и функциональная диагностика 2014; 4: 41—46. (Sirotina O.B., Zubareva E.A. Ultrasound evaluation of the thymus fetuses and newborns of mothers with Type 1 diabetes. Ul’trazvukovaja i funkcional’naja diagnostika 2014; 4: 41—46.)
4. Сорокина И.В., Куприянова Л.С. Иммунофенотипические особенности тимусов доношенных плодов от матерей, страдающих сахарным диабетом 1-го типа. Патологія 2007; 4: 1: 47—50. (Sorokina I.V., Kuprijanova L.S. Immunophenotypic features thymuses term fetuses of mothers with Type 1 diabetes. Patologіja 2007; 4: 1: 47—50.)
5. Куприянова Л.С. Особенности интерлейкинпродуцирующей активности тимуса плодов с признаками задержки внутриутробного развития от матерей, страдающих сахарным диабетом I типа. Морфологiя 2007; 1: 4: 116—117. (Kuprijanova L.S. Features interleukin-producing activity of the thymus of fetuses with intrauterine growth characteristics of mothers with diabetes mellitus type I. Morfologija 2007; 1: 4: 116—117.)
6. Roll U., Scheeser J, Standl E., Ziegler A.G. Alterations of lymphocyte subsets in children of diabetic mothers. Diabetologia. 1994; 37: 11:1132—1341.
7. Holm B.C., Svensson J., Akesson C. et al. Evidence for immunological priming and increased frequency of CD4+ CD25+ cord blood T cells in children born to mothers with type 1 diabetes. Clin Exp Immunol 2006; 146: 3:493—502.
8. Franзa E.L., Calderon I.M. Paranhos, Vieira E. L. et al. Transfer of Maternal Immunity to Newborns of Diabetic Mothers Clin Dev Immunol. 2012; 2012: 928187.
9. Колесникова Н.В., Мустафа М., Никулин Л.А. Иммунотропные эффекты ликопида при нарушениях микробицидной функции нейтрофилов новорожденных с диабетической фетопатией. Педиатрия 2006; 1: 79—83. (Kolesnikova N.V., Mustafa M., Nikulin L.A. Immunotropic effects licopid for violations of the microbicidal function of neutrophils of newborns with diabetic fetopathy. Pediatrija 2006; 1: 79—83.)
10. Yessoufou A., Moutairou K. Maternal Diabetes in Pregnancy. Early and Long-Term Outcomes on the Offspring and the Concept of Metabolic Memory. Experimental Diabetes Research 2011; 2011:1—12.
11. Дедов И.И., Краснопольский В.И., Сухих Г.Т. Российский национальный консенсус «Гестационный сахарный диабет: диагностика, лечение, послеродовое наблюдение». Сахарный диабет 2012; 4: 4—10. (Dedov I.I., Krasnopol’skij V.I., Suhih G.T. The Russian national consensus «Gestational diabetes: diagnosis, treatment, postnatal care». Saharnyj diabet 2012; 4: 4—10.)
12. Хаитов Р.М. Иммунология. + CD. М: ГЭОТАР-Медиа 2006; 320. (Haitov R.M. Immunology. + CD. M.: GJeOTAR- Media 2006; 320.)
Review
For citations:
Chistyakova G.N., Remizova I.I., Gazieva I.A., Lyapunov V.A., Ustyantseva L.S. Features of the immune system of children born to women with gestational diabetes mellitus. Rossiyskiy Vestnik Perinatologii i Pediatrii (Russian Bulletin of Perinatology and Pediatrics). 2015;60(2):42-48. (In Russ.)
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