Specific features of immunity in neonatal infants with localized and generalized bacterial infections
Abstract
Cellular immunity indicators were investigated in infants with localized (и=10) and generalized (и=15) bacterial infections (sepsis). The blood levels of different groups of white blood cells (neutrophils, lymphocytes, monocytes) and lymphocyte subpopulations (CD3, CD4, CD8, CD16/56, and CD19) were estimated. The development of sepsis was accompanied by leukocytosis and leukopenia in 47 and 20% of the cases, respectively. In localized infections, there was leukocytosis in 30% of the infants and leukopenia in 10%. Absolute lymphopenia was present in 40% of the cases of sepsis and in 20% of those of localized infections. The acute period of neonatal sepsis was accompanied by a substantial reduction in the level of CD3+, CD4+, CD8+, and CD16/56+ lymphocyte subpopulations. The highest changes were recorded in the CD4+ and CD16/56+: their counts were decreased by an average of 3,9 and 3,6 times, respectively, compared to the control values; CD8+ lymphocytes dropped by 2,7 times. Localized infections were characterized by a predominant fall in the number of CD4+ and CD16/56+ subpopulations. Moreover, the level of CD4+ lymphocytes was significantly lower than that in the sepsis group. Thus, the acute period of both localized and generalized bacterial infections took place in the presence of cellular immunity suppression. The most marked changes were recorded in sepsis. The fi
About the Authors
Kh. S. Khaertynov
Kazan State Medical University
Russian Federation
Russian Federation
V. A. Anokhin
Kazan State Medical University
Russian Federation
Russian Federation
I. G. Mustafin
Kazan State Medical University
Russian Federation
Russian Federation
S. V. Boichuk
Kazan State Medical University
Russian Federation
Russian Federation
M. A. Satrutdinov
Republican Children's Clinical Hospital
Russian Federation
Russian Federation
A. A. Andreeva
Republican Children's Clinical Hospital
Russian Federation
Russian Federation
S. A. Lyubin
City Children's Hospital One, Kazan
Russian Federation
Russian Federation
I. V. Agapova
City Children's Hospital One, Kazan
Russian Federation
Russian Federation
References
1. Lawn J.E., Cousens S., Zupan J.T. Lancet Neonatal Survival Steering. 4 million neonatal deaths: when? Where? Why? Lancet 2005; 365: 9462: 891-900.
2. Атауллаханов Р.И., Гшщбург А.Л. Иммунитет и инфекция: динамичное противостояние живых систем. Педиатрия 2005; 4: 47—61. (Ataullahanov R.I., Gincburg A.L. Immunity and dynamic confrontation of living systems. Pediatriya2005; 4: 47-61.)
3. Самсыгина Г.А. О предрасполагающих факторах и факторах риска развития неонатального сепсиса и о современных подходах его лечения. Педиатрия 2012; 91: 3: 32—37. (Samsygina G.A. About predisposing factors and risk factors for neonatal sepsis and modern approaches its treatment. Pe-diatriya2012; 91: 3:32-37.)
4. Melville J.M., Moss L.J.M. The immune consequences of preterm birth. Front Neurosci 2013; 7: doi: 10.3389/ fnins.2013.00079
5. ХаертыновХ.С., БойчукС.В., АнохинВ.А. идр. Показатели активности апоптоза лимфоцитов крови у детей с неонатальным сепсисом. Гены и клетки 2014; 9: 3: 267—271. (Khaertynov H.S., Boichuk S.V., Anohin VA. et al. Activity rates of apoptosis of lymphocytes in children with neonatal sepsis. Geny ikletki2014; 9: 3: 267-271.)
6. Correa-Rocha R., Perez A., Lorente R. et al. Preterm neonates show marked leucopenia and lymphopenia that are associated with increased regulatory T-cell values and diminished IL-7. PediatRes 2012; 71: 5: 590-597.
7. Hotchkiss R.S., Monneret G., Payen D. Sepsis-induced immu-nosuppression: from cellular dysfunctions to immunotherapy. Immunology 2013; 13: 862-874.
8. Romani L. Immunity to fungal infections. Nature Rev Immunol 2011; 11:275-288.
9. Souza-Lonseca-Guimaraes E, Parlato M., Lifting С et al. NK cell tolerance to TLR agonists mediated by regulatory T cells after polymicrobial sepsis. J Immunol 2012; 188: 5850-5858.
10. Самсыгина L.A. Неонатальный сепсис. М: Союз педиатров России 2014; 173. (Samsygina GA. Neonatal sepsis. Moscow: SoyuzpediatrovRossii2014; 173.)
11. van den Berg J. P., WesterbeekE.A.M., van derKlisL.R.M. etal. Transplacental transport of IgG antibodies to preterm infants:
12. a review of the literature. Early Hum 2011; 87: 67—72.
13. Drifte G., Dunn-Siegrist L., Lissieres P. et al. Innate immune functions of immature neutrophils in patients with sepsis and severe systemic inflammatory response syndrome. Crit Care Med2013; 41: 820-832.
14. Boomer J.S., Lo K., Chang K.C. Immunosuppression in Patients Who Die of Sepsis and Multiple Organ Failure. JAMA 2011; 306: 23: 2594-2605.
15. Hotchkiss R.S., Linsley K.W., Swanson P.E. et al. Sepsis-induced apoptosis causes progressive profound depletion of В and CD4+ T lymphocytes in humans. J Immunol 2001; 166: 6952-6963.
16. Hotchkiss R.S., Linsley K.W., Swanson P.E. etal. Depletion of dendritic cells, but not macrophages, in patients with sepsis. J Immunol 2002; 168: 2493-2500.
17. Huang L.E, Yao Y.M., Dong N. et al. Association between regulatory T cell activity and sepsis and outcome of severely burned patients: a prospective, observational study. Crit Care 2010; 14: R3.
18. Venet E, Pachot A., Debard A.L. et al. Human CD4+CD25+ regulatory T lymphocytes inhibit lipopolysaccharide-induced monocyte survival through a Fas/Fas ligand-dependent mechanism. J Immunol 2006; 177: 6540-6547.
19. EelmetKA., Hall M.W., Clark R.S. etal. Prolonged lymphopenia, lymphoid depletion, and hypoprolactinemia in children with nosocomial sepsis and multiple organ failure. J Immunol 2005; 174: 3765-3772.
20. Hotchkiss R.S., Swanson P.E., Freeman B.D. et al. Apoptotic cell death in patients with sepsis, shock, and multiple organ dysfunction. Crit Care Med 1999; 27: 1230-1251.
21. Loti P., Be Felice C, Occhini R. et al. Spleen depletion in neonatal sepsis and chorioamnionitis. Am J Clin Pathol 2004; 122: 765-771.
22. Hotchkiss R.S., SchmiegR.L., Swanson P.E. etal. Rapid onset of intestinal epithelial and lymphocyte apoptotic cell death in patients with trauma and shock. Crit Care Med 2000; 28: 3207-3217.
23. Kasten K.R., Lschop J., Adediran S.G. et al. T cells are potent early mediators of the host response to sepsis. Shock 2010; 34: 4: 327-336.
Review
For citations:
Khaertynov Kh.S., Anokhin V.A., Mustafin I.G., Boichuk S.V., Satrutdinov M.A., Andreeva A.A., Lyubin S.A., Agapova I.V. Specific features of immunity in neonatal infants with localized and generalized bacterial infections. Rossiyskiy Vestnik Perinatologii i Pediatrii (Russian Bulletin of Perinatology and Pediatrics). 2015;60(5):168-173. (In Russ.)
Views:
788
Email this article 